The AutoTune should be on all the time to function properly. It is constantly tuning depending on all the varibles and the o2 sensor inputs. It is also recording the adjustments it is making in each fuel Map cell. For the first several rides, you should "Accept Trims" in the AutoTune software which adjusts the base Map to what the AutoTune is correcting. Once the trims have been accepted a few times the base Map is nearly perfect but the AutoTune will always keep making tiny adjustments. The number values you see in the Trims are percentages, + or - of change to the fuel map in the ECU or the fuel map in the PC V once trims are accepted. A number of 10 would be adding 10 percent fuel in that cell (specific rpm and throttle position). Even if you never accept trims, as long as the base map is close the AutoTune will do the rest and make the adjustments. Looking at the trims will tell you how close the current map is to what the AutoTune is adjusting. The first several rides you may see larger trim numbers, as high as 40 or so. Once trims are accepted a few time you will begin to see much smaller numbers 1, 2 , 3 etc., meaning the AutoTune is making minute adjustments since the map is so close. If you don't leave the AutoTune on full time it doesn't make a lot of sense to have it since it can't adjust the map. It is an excellent product and works great. In the cool spring and fall, I see plus numbers as the AutoTune adds a little fuel due to cooler air temps - would add even more in cold winter temps. In the hot summer I see negative numbers as the AutoTune takes a little fuel out.