I assumed you double checked cam timing (alignment marks). Broken injector wire? Describe the mis-fire. Pop through the exhaust, no combustion at all, pop through intake, etc.
2007 Street Glide
Hobanized Crank, Axtell 117" cylinders, CP flat tops, 10.6:1 CR, Woods TW8, R&R cast heads, R&R roller rockers, HPI 51mm T/B, 4.9 g/s SE injectors, D&D Fat Cat 2into1 QB, Rivera Pro Clutch, Evo Industries 49T, Tuned by Big Boyz